Dr. Charest's Publications (link to PubMed)
22. AFM T. Islam, M. Scavello M., P. Lotfi, D. Daniel, P. Haldeman, P.G. Charest. Caffeine inhibits PI3K and mTORC2 in Dictyostelium and differentially affects multiple other cAMP chemoattractant signaling effectors. Mol. Cell. Biochem. (2019) In press. doi: 10.1007/s11010-019-03520-z
21. AFM T. Islam, H. Yue, M. Scavello, P. Haldeman, W.J. Rappel, and P.G. Charest. The cAMP-induced G protein subunits dissociation monitored in live Dictyostelium cells by BRET reveals two activation rates, a positive effect of caffeine and potential role of microtubules. Cell. Signal. 48:25-37 (2018). pdf
20. M. Scavello*, A.R. Petlick*, R. Ramesh, V.F. Thompson, P. Lotfi, and P.G. Charest. Protein Kinase A regulates Ras, Rap1, and TORC2 pathways in response to the chemoattractant cAMP in Dictyostelium. J. Cell Sci. 130:1545-1558 (2017). pdf
19. AFM T. Islam, B.M. Stepanski, and P.G. Charest. Studying chemoattractant signal transduction dynamics in Dictyostelium by BRET. Meth. Mol. Biol. 1407:63-77 (2016). pdf
18. A. Khanna*, P. Lotfi*, A.J. Chavan, N.M. Montaño, P. Bolourani, G. Weeks, Z. Shen, S.P. Briggs, H. Pots, P.J.M Van Haastert, A. Kortholt, and P.G. Charest. The small GTPases Ras and Rap1 bind to and control TORC2 activity. Sci. Rep. 6:25823 (2016). *equal contribution. pdf
17. K. Sumita, H. Yoshino, M. Sasaki, N. Majd, E.R. Kahoud, H. Takahashi, K. Takeuchi, T. Kuroda, S. Lee, P.G. Charest, K. Takeda, J.M. Asara, R.A. Firtel, D. Anastasiou, and A.T. Sasaki. Degradation of activated K-Ras orthologue via K-Ras specific lysine residues is required for cytokinesis. J. Biol. Chem. 289:3950-9 (2014). pdf
16. V. Kölsch, Z. Shen, S. Lee, K. Plak, P. Lotfi, J. Chang, P.G. Charest, J.L. Romero, T.J. Jeon, A. Kortholt, S.P. Briggs, and R.A. Firtel. Daydreamer, a Ras effector and GSK-3 substrate, is important for directional sensing and cell motility. Mol. Biol. Cell. 24(2):100-14 (2013). pdf
15. K. Takeda, D. Shao, M. Adler, P.G. Charest, W.F. Loomis, H. Levine, A. Groisman, W.J. Rappel, and R.A. Firtel. Incoherent feedforward control governs adaptation of activated Ras in eukaryotic chemotaxis pathway. Sci. Signal. 5, ra2 (2012). pdf
14. I. Hecht, M.L. Skoge, P.G. Charest, E. Ben-Jacob, R.A. Firtel, W.F. Loomis, H. Levine, and W.J. Rappel. Activated membrane patches guide chemotactic cell motility. PLoS Comput. Biol. 7(6):e1002044 (2011). pdf
13. P.G. Charest, Z. Shen, A. Lakoduk, A.T. Sasaki, S.P. Briggs, and R.A. Firtel. A Ras signaling complex controls the RasC-TORC2 pathway and directed cell migration. Dev. Cell. 18:737-49 (2010). pdf
12. P.G. Charest and R.A. Firtel. “TORCing” neutrophil chemotaxis. Dev. Cell 19(6):795-6 (2010). pdf
11. S. Zhang*, P.G. Charest*, and R.A. Firtel. Spatio-temporal Regulation of Ras Activity Provides Directional Sensing. Curr. Biol. 18(20):1587-93 (2008). * Equal authorship. pdf
10. V. Kölsch, P.G. Charest, and R.A. Firtel. The regulation of cell motility and chemotaxis by phospholipid signaling. J. Cell Sci. 121(Pt 5):551-9 (2008). pdf
9. F.F. Hamdan, M.D. Rochdi, B. Breton, D. Fessart, D.E. Michaud, P.G. Charest, S.A. Laporte, and M. Bouvier. Unraveling g protein-coupled receptor endocytosis pathways using real-time monitoring of agonist-promoted interaction between beta-arrestins and AP-2. J. Biol. Chem. 282(40):29089-100 (2007). pdf
8. A.T. Sasaki, C. Janetopoulos, S. Lee, P.G. Charest, K. Takeda, L.W. Sunddheimer, R. Meili, P.N. Devreotes and R.A. Firtel. G Protein-Independent Ras/PI3K/F-Actin Circuit Regulates Basic Cell Motility. J. Cell. Biol. 178(2):185-91 (2007). pdf
7. P.G. Charest and R.A. Firtel. Big roles for small GTPases in the control of directed cell movement. Biochem. J. 401(2):377-90 (2007). pdf
6. P.G. Charest, G. Oligny-Longpré, H. Bonin, M. Azzi and M. Bouvier. The V2 vasopressin receptor stimulates ERK1/2 activity independently of heterotrimeric G protein signalling. Cell. Signal. 19(1):32-41 (2007). pdf
5. P.G. Charest and R.A. Firtel. Feedback signaling controls leading edge formation during chemotaxis. Curr. Opin. Genet. Dev. 16(4):339-47 (2006). pdf
4. P.G. Charest, S. Terrillon and M. Bouvier. Monitoring agonist-promoted conformational changes of b-arrestin in living cells by intramolecular BRET. EMBO rep. 6(4): 334-40 (2005). pdf
3. J. Perroy, S. Pontier, P.G. Charest, M. Aubry and M. Bouvier. Real-time monitoring of ubiquitination in living cells by BRET. Nat. Meth. 1(3):203-8 (2004). pdf
2. P.G. Charest and M.Bouvier. Palmitoylation of the V2 vasopressin receptor carboxyl tail facilitates barrestin recruitment leading to efficient receptor endocytosis and ERK1/2 activation. J. Biol. Chem. 278(42):41541-51 (2003). pdf
1. M. Azzi, P.G. Charest, S. Angers, M. Bouvier and G. Pineyro. bArrestin-mediated activation of MAPK by inverse agonists reveals distinct active conformations for GPCRs. Proc. Natl. Acad. Sci. U S A 100(20):11406-11 (2003). pdf
21. AFM T. Islam, H. Yue, M. Scavello, P. Haldeman, W.J. Rappel, and P.G. Charest. The cAMP-induced G protein subunits dissociation monitored in live Dictyostelium cells by BRET reveals two activation rates, a positive effect of caffeine and potential role of microtubules. Cell. Signal. 48:25-37 (2018). pdf
20. M. Scavello*, A.R. Petlick*, R. Ramesh, V.F. Thompson, P. Lotfi, and P.G. Charest. Protein Kinase A regulates Ras, Rap1, and TORC2 pathways in response to the chemoattractant cAMP in Dictyostelium. J. Cell Sci. 130:1545-1558 (2017). pdf
19. AFM T. Islam, B.M. Stepanski, and P.G. Charest. Studying chemoattractant signal transduction dynamics in Dictyostelium by BRET. Meth. Mol. Biol. 1407:63-77 (2016). pdf
18. A. Khanna*, P. Lotfi*, A.J. Chavan, N.M. Montaño, P. Bolourani, G. Weeks, Z. Shen, S.P. Briggs, H. Pots, P.J.M Van Haastert, A. Kortholt, and P.G. Charest. The small GTPases Ras and Rap1 bind to and control TORC2 activity. Sci. Rep. 6:25823 (2016). *equal contribution. pdf
17. K. Sumita, H. Yoshino, M. Sasaki, N. Majd, E.R. Kahoud, H. Takahashi, K. Takeuchi, T. Kuroda, S. Lee, P.G. Charest, K. Takeda, J.M. Asara, R.A. Firtel, D. Anastasiou, and A.T. Sasaki. Degradation of activated K-Ras orthologue via K-Ras specific lysine residues is required for cytokinesis. J. Biol. Chem. 289:3950-9 (2014). pdf
16. V. Kölsch, Z. Shen, S. Lee, K. Plak, P. Lotfi, J. Chang, P.G. Charest, J.L. Romero, T.J. Jeon, A. Kortholt, S.P. Briggs, and R.A. Firtel. Daydreamer, a Ras effector and GSK-3 substrate, is important for directional sensing and cell motility. Mol. Biol. Cell. 24(2):100-14 (2013). pdf
15. K. Takeda, D. Shao, M. Adler, P.G. Charest, W.F. Loomis, H. Levine, A. Groisman, W.J. Rappel, and R.A. Firtel. Incoherent feedforward control governs adaptation of activated Ras in eukaryotic chemotaxis pathway. Sci. Signal. 5, ra2 (2012). pdf
14. I. Hecht, M.L. Skoge, P.G. Charest, E. Ben-Jacob, R.A. Firtel, W.F. Loomis, H. Levine, and W.J. Rappel. Activated membrane patches guide chemotactic cell motility. PLoS Comput. Biol. 7(6):e1002044 (2011). pdf
13. P.G. Charest, Z. Shen, A. Lakoduk, A.T. Sasaki, S.P. Briggs, and R.A. Firtel. A Ras signaling complex controls the RasC-TORC2 pathway and directed cell migration. Dev. Cell. 18:737-49 (2010). pdf
12. P.G. Charest and R.A. Firtel. “TORCing” neutrophil chemotaxis. Dev. Cell 19(6):795-6 (2010). pdf
11. S. Zhang*, P.G. Charest*, and R.A. Firtel. Spatio-temporal Regulation of Ras Activity Provides Directional Sensing. Curr. Biol. 18(20):1587-93 (2008). * Equal authorship. pdf
10. V. Kölsch, P.G. Charest, and R.A. Firtel. The regulation of cell motility and chemotaxis by phospholipid signaling. J. Cell Sci. 121(Pt 5):551-9 (2008). pdf
9. F.F. Hamdan, M.D. Rochdi, B. Breton, D. Fessart, D.E. Michaud, P.G. Charest, S.A. Laporte, and M. Bouvier. Unraveling g protein-coupled receptor endocytosis pathways using real-time monitoring of agonist-promoted interaction between beta-arrestins and AP-2. J. Biol. Chem. 282(40):29089-100 (2007). pdf
8. A.T. Sasaki, C. Janetopoulos, S. Lee, P.G. Charest, K. Takeda, L.W. Sunddheimer, R. Meili, P.N. Devreotes and R.A. Firtel. G Protein-Independent Ras/PI3K/F-Actin Circuit Regulates Basic Cell Motility. J. Cell. Biol. 178(2):185-91 (2007). pdf
7. P.G. Charest and R.A. Firtel. Big roles for small GTPases in the control of directed cell movement. Biochem. J. 401(2):377-90 (2007). pdf
6. P.G. Charest, G. Oligny-Longpré, H. Bonin, M. Azzi and M. Bouvier. The V2 vasopressin receptor stimulates ERK1/2 activity independently of heterotrimeric G protein signalling. Cell. Signal. 19(1):32-41 (2007). pdf
5. P.G. Charest and R.A. Firtel. Feedback signaling controls leading edge formation during chemotaxis. Curr. Opin. Genet. Dev. 16(4):339-47 (2006). pdf
4. P.G. Charest, S. Terrillon and M. Bouvier. Monitoring agonist-promoted conformational changes of b-arrestin in living cells by intramolecular BRET. EMBO rep. 6(4): 334-40 (2005). pdf
3. J. Perroy, S. Pontier, P.G. Charest, M. Aubry and M. Bouvier. Real-time monitoring of ubiquitination in living cells by BRET. Nat. Meth. 1(3):203-8 (2004). pdf
2. P.G. Charest and M.Bouvier. Palmitoylation of the V2 vasopressin receptor carboxyl tail facilitates barrestin recruitment leading to efficient receptor endocytosis and ERK1/2 activation. J. Biol. Chem. 278(42):41541-51 (2003). pdf
1. M. Azzi, P.G. Charest, S. Angers, M. Bouvier and G. Pineyro. bArrestin-mediated activation of MAPK by inverse agonists reveals distinct active conformations for GPCRs. Proc. Natl. Acad. Sci. U S A 100(20):11406-11 (2003). pdf